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Catalog#/Size：AC52388/100 μg.

Source：Escherichia coli.

Molecular Weight：Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 134 amino acid.

Description：Accession # P60658.1, Ala21-Thr153（C125A）, with an N-terminal Ser.

SDS-PAGE：15.5kDa, reducing conditions

Purity：> 95 %, as determined by SDS-PAGE, under reducing non-reducing conditions, visualized by coomassie staining.

Endotoxin：Less than 0.01 EU/μg of rHuIL-2 as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay.

Biological Activity：Recombinant human IL-2 bioactivity is measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells, the EC50 for this effect is 5.214 to 6.755 ng/mL.


Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation：Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB pH 3.5 with 10% trehalose anhydrous.
Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute to a concentration of 0.1-1.0 mg/mL in sterile distilled H2O. Stock solutions should be apportioned into working aliquots and stored at-20 ℃ to -70 ℃. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly.
Shipping：The product is shipped at 2 °C to 8 °C. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
             A minimum of 12 months from date of shipping when stored at -20℃ to -70℃ as supplied.
             4 weeks at 2 ℃ to 8 ℃ under sterile conditions after reconstitution.
             4 months at -20 ℃ to -70 ℃ under sterile conditions after reconstitution.
Usage：Acnovia rHuIL-2 product can be used for a variety of ex vivo cell culture applications such as research or further manufacturing.

Quality statement:No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin.


IL-2, also named T-cell growth factor, was first discovered in 1976 and was characterized as a soluble factor with the unique ability to promote clonal expansion of many hematopoietic lines including regulatory T cells (Tregs) in vitro. Human IL-2 has 133 amino acids and in mature form it is a glycosylated globular protein of 15.5 k Da four-bundle,  α  -helical protein member of the common cytokine receptor  γ  -chain family of cytokines. IL-2 is predominantly produced by activated CD4+ T cells and, to a lesser extent by activated CD8+ T cells, activated dendritic cells, natural killer (NK) cells, NKT cells, as well as B cells.

1. Liao W, et al. 2013. Immunity. 38:13-25.

2. Sakaguchi S, et al. 2008. Cell. 133:775-87.

3. Kryczek, I. et al. 2007. J. Immunol. 178:6730.

4. Cote-Sierra J, et al. 2004. Proc Natl Acad Sci USA. 101:3880-5.


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