Catalog#/Size：GMP-AC52387/100 μg.
Source：Escherichia coli.
Molecular Weight：Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids.
Description：Accession # P13232.1, Asp26-His177, with an N terminal Met.
SDS-PAGE： 17 kDa, reducing conditions.
Purity：> 95 %, as determined by SDS-PAGE, under reducing non-reducing conditions, visualized by coomassie staining and > 95% as determined by RP-HPLC.
Endotoxin：Less than 0.01 EU/μg of GMP Recombinant Human IL-7 as determined by kinetic Limulus Amoebocyte Lysate (LAL) assay.
Sterility：The sterility testing was performed by Direct inoculation Method described in CP<1101>.
Biological Activity：Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes. The EC50 for this effect is 4.017-4.985 ng/mL. The specific activity of GMP Recombinant Human IL-7 is>1.0x10⁸ IU/mg, which is calibrated against human IL-7 WHO International Standard (NIBSC code: 90/530).

Host Cell Protein：< 0.5 ng per μg of protein when tested by ELISA.
Host Cell DNA：< 0.0015 ng per μg of protein when tested by qPCR.
Kanamycin：Negative when tested by ELISA.
Physical Appearance：Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute to a concentration of 0.1-1.0 mg/mL in sterile distilled H₂O. Stock solutions should be apportioned into working aliquots and stored at -20℃ to -70℃. Further dilutions should be made in appropriate buffered solutions. Do not reconstitute in cell culture media directly.
Shipping：The product is shipped at 2℃ to 8℃. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
A minimum of 12 months from date of shipping when stored at -20℃ to -70℃ as supplied.
4 weeks at 2℃ to 8℃ under sterile conditions after reconstitution.
4 months at -20℃ to -70℃ under sterile conditions after reconstitution.
Usage：Acnovia rHuIL-7 product can be used for a variety of ex vivo cell culture applications such as research or further manufacturing.
Quality statement：No animal- or human-derived materials were used for the manufacture of this product, unless otherwise stated in the respective Certificate of Origin.

Interleukin-7(IL-7) is a soluble globular glycoprotein of about 25 kDa (152 amino acids in humans) and encoded by the gene that located on chromosome 8q12-13. Human IL-7 cDNA encodes 177 amino acids (aa) which contain a 25 aa signal peptide. Human IL-7 exhibits about 60-76% aa sequense with mouse, rat, equine, bovine, ovine, porcine, feline and canine IL-7. Human and mouse IL-7 has the species-crossing activity. IL-7 is produced by a wide variety of cells, such as fetal liver cells, stromal cells in the bone marrow (BM), and thymus and other epithelial cells, including keratinocytes and enterocytes. The IL-7 receptor(IL-7R) is a heterodimeric complex consisting of the α-chain (CD127) and the common cytokine receptor γ-chain which is shared with IL-2, IL-4, IL-7, IL-9, IL-15, and IL-21, and expressed in a variety of cells. IL-7 plays a vital role in T-cell development, proliferation, and differentiation, as well as in B cell maturation through the activation of the IL-7 receptor (IL-7R). IL-7 is a highly pleiotropic cytokine that is required for the efficient generation of lymphocytes from HSCs and maintains the survival of B and T cells by regulating B-cell lymphoma-2 (Bcl-2) family proteins and providing proliferation signals to these lymphocytes. Other than that IL-7 is primarily involved in regulating the development of B cells, T cells, natural killer cells, and dendritic cells via the JAK-STAT, PI3K-Akt, and MAPK pathways.

1.Barata JT, Silva A, Abecasis M, Carlesso N, Cumano A, Cardoso AA. Molecular and functional evidence for activity of murine IL-7 on human lymphocytes. Exp Hematol. 2006 Sep;34(9):1133-42.

2.Chen D, Tang TX, Deng H, Yang XP, Tang ZH. Interleukin-7 Biology and Its Effects on Immune Cells: Mediator of Generation, Differentiation, Survival, and Homeostasis. Front Immunol. 2021 Dec 2;12:74732.